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KMID : 0379119930210010028
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Korean Journal of Mycology
1993 Volume.21 No. 1 p.28 ~ p.37
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Purification and Characterization of Carboxymethyl Cellulase 4 from Penicillium verruculosum
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Abstract
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An endo-type cellulase, carboxcymethyl cellulase(CMCase) IV, was purified from culture filtrate of cellulolytic fungus Penicillium verruculosum. The CMCase IV was acidic glycoprotein having carbohydrate of 13% as glucose and pI value of 4.0. The CMCase IV was 52 KDa of molecular weight in SDS-polyacrylamide gel electrophoresis and have optimum temperature and pH of 50℃ and 5.0 for enzyme activity. The CMCase IV liberated glucose and cellobiose as major produced of the enzyme against carboacymethyl cellulose(CMC) and seemed to has transglycosylation activity simultaneously. Cellulase activity staining(zymogram) showed that the cellulase components of P. verruculosum were not aggregated in the medium. P. verruculosum mRNA was translated in vitro and translation product by the mRNA coding for CMCase IV was identified by immunoprecipitation.
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